| 產(chǎn)品名稱 | 
      Ray Hot | 
    
    
      | 商品貨號(hào) | 
      B162307 | 
    
      
      | Organism | 
      Homo sapiens, human | 
    
      
      | Tissue | 
      skin | 
    
      
      | Product Format | 
      frozen | 
    
      
      | Morphology | 
      fibroblast | 
    
      
      | Culture Properties | 
      adherent | 
    
      
      | Biosafety Level | 
      1 
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.   | 
    
      
      | Disease | 
      Ehlers-Danlos syndrome, type III | 
    
      
      | Age | 
      23 years | 
    
      
      | Gender | 
      male | 
    
      
      | Clinical Data | 
      The tissue donor suffered from type III Ehlers-Danlos syndrome, and was the son of Ad Hot (ATCC CRL-1227) who was affected with the same condition. male  | 
    
      
      | Comments | 
      The tissue donor suffered from type III Ehlers-Danlos syndrome, and was the son of Ad Hot (ATCC CRL-1227) who was affected with the same condition.  | 
    
      
      | Complete Growth Medium | 
      The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.  To make the complete growth medium, add the following components to the base medium:  fetal bovine serum to a final concentration of 10%.
        
  | 
    
      
      | Subculturing | 
      Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.  |