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    Human adenovirus 5
    Human adenovirus 5
    規(guī)格:
    貨期:
    編號:B162881
    品牌:Mingzhoubio

    標準菌株
    定量菌液
    DNA
    RNA

    規(guī)格:
    凍干粉
    斜面
    甘油
    平板


    產(chǎn)品名稱 Human adenovirus 5
    商品貨號 B162881
    Classification Adenoviridae, Mastadenovirus, Human adenovirus C
    Deposited As Adenovirus type 5
    Agent Human adenovirus 5
    Strain Adenoid 75
    Applications
    Viruscide testing
    Respiratory research
    Biosafety Level 2

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Product Format frozen 1 mL per vial
    Storage Conditions -70°C or colder
    Comments Not inactivated by ether. Contains soluble group-specific complement fixation antigen. Agglutinates rat red blood cells incompletely.
    Effect on Host
    CPE, refractile rounding, clumping and sloughing
    Recommended Host
    HeLa (ATCC CCL-2)
    Growth Conditions Temperature: 37°C
    Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
    Incubation: 2-3 days
    Name of Depositor RJ Huebner, National Institutes of Health; M Shaffer, Public Health Research Institute of the City of New York
    Special Collection NCRR Contract
    Source Spontaneously degenerating tissue culture of adenoid tissue from a 4-year-old girl with enlarged chronically infected tonsils, Washington DC, 1953
    References

    Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK:British Standards Institution;British Standard BS EN 14885:2006.

    Chemical disinfectants and antiseptics --- Virucidal quantitative suspension test for chemical disinfectants and antiseptics used in human medicine --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 14476:2005.

    Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

    Huebner RJ, et al. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc. Soc. Exp. Biol. Med. 84: 570-573, 1953. PubMed: 13134217

    Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

    Rowe WP, et al. Studies of the adenoidal-pharyngeal-conjunctival (APC) group of viruses. Am. J. Hyg. 61: 197-218, 1955.

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