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    pCNV220
    pCNV220
    規(guī)格:
    貨期:
    編號(hào):B233667
    品牌:Mingzhoubio

    標(biāo)準(zhǔn)菌株
    定量菌液
    DNA
    RNA

    規(guī)格:
    凍干粉
    斜面
    甘油
    平板


    產(chǎn)品名稱 pCNV220
    商品貨號(hào) B233667
    Designations pCNV220
    GenBank Number

    M25270

    Species Cucumber necrosis virus (CNV)
    Depositors DM Rochon
    Applications
    contains sequence genome, 90%+ complete
    Vector
    Construct size (kb): 7.5
    Insert
    DNA: cDNA
    Insert lengths(kb): 4.699999809265137
    Gene product: genome, 90%+ complete
    Target Gene: genome, 90%+ complete
    Insert Size (kb) 4.700
    Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
    Biosafety Level 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Comments
    Restriction digests of the clone give the following sizes (kb): PstI--4.6, 3.0; HindIII--3.5, 1.4, 1.3, 1.2; BglI--2.7, 1.7, 1.5 (doublet).
    Insert contains the following restriction sites (approximate kb from the 5' end): BglI--2.66, 4.29; BglII--3.36; EcoRI--2.14; EcoRV--1.07, 1.49, 1.57, 3.19, 4.03; HindIII--0.62, 1.80, 3.22; KpnI--3.89; NdeI--0.64, 1.58, 3.52, 3.63; PvuII--2.49, 3.33.
    Contains entire coding sequence plus 20 nucleotides of 5' noncoding sequence.
    Virus was isolated from cucumber, Cucumis sativus (cultivar strain 8).
    Classification Tombusvirus
    References

    Rochon DM, Tremaine JH. Complete nucleotide sequence of the cucumber necrosis virus genome. Virology 169: 251-259, 1989. PubMed: 2705296

    D'Ann M Rochon, personal communication

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